Alkaline Phosphatase Assay Kit 

堿性磷酸酶活性檢測試劑盒（顯色法）

【溫馨提示I】：見堿性磷酸酶產(chǎn)品專題，了解更多相關產(chǎn)品資料；

【溫馨提示II】：見堿性磷酸酶染色試劑盒產(chǎn)品；

【溫馨提示III】：見堿性磷酸酶常用生化底物及試劑盒產(chǎn)品；

 產(chǎn)品關鍵詞：

Phosphatase, Alkaline堿性磷酸酶；ALP/AKP；對硝基苯酚磷酸鹽（pNPP）；偶氮偶聯(lián)法；顯色法（定量）；染色法（定性）； 

基本介紹：

? 堿性磷酸酶（Phosphatase, Alkaline，ALP/AP/AKP/ALKP/ALPase，EC 3.1.3.1），是一種含兩個相同亞基的二聚體，也是一種約含12%碳水化合物（6%己糖和6%其他中性糖）的糖蛋白，每個ALP分子含4個鋅離子和4個二硫鍵。鎂離子存在情況下，ALP可達到zui高酶活。堿性條件下，ALP能催化磷酸單酯水解，比如對硝基苯酚磷酸鹽（pNPP）、磷酸苯酯、葡萄糖-6-磷酸、葡萄糖-1-磷酸、果糖-6-磷酸、腺苷-5-磷酸等。

? 常見的堿性磷酸酶ALP包括腸道堿性磷酸酶（如小牛腸來源，calf intestinal）、胎盤堿性磷酸酶（placental alkaline phosphatase）、非組織特異性堿性磷酸酶（tissue-nonspecific isozyme, alkaline phosphatase）等。

? ALP常用來偶聯(lián)抗體和其他蛋白，從而滿足ELISA、WB和組織化學檢測上的應用。通常用來蛋白的去磷酸化實驗，比如酪蛋白和核酸等。當需要高檢測靈敏度時也能用來蛋白標記。還可用在DNA或RNA 的5′-末端去磷酸以防止自連。經(jīng)堿性磷酸酶的去磷酸化作用后，DNA 或 RNA 還可被放射性標記磷酸鹽標記（通過 T4 多核苷酸激酶）。
 

研究意義：
堿性磷酸酶（ALP）活性的變化與一系列生理和病理事件緊密關聯(lián)，比如骨骼發(fā)育、骨骼相關疾病、ren娠相關疾病和炎癥性腸病（inflammatory bowel diseases）等。正因如此，ALP被用作衡量生理特征或疾病的參照指標。另外，ALP活性檢測在環(huán)境監(jiān)測、食品質量控制等方面也呈現(xiàn)重要作用。? 干細胞如誘導多潛能干細胞iPS，ALP活性很高，則ALP常被用作iPS誘導成功的標志；? 分化的結腸癌細胞內ALP活性顯著提高，則ALP常被當做結腸癌細胞分化程度的指標（定性/定量）；? 血清中ALP水平提高，被稱作高堿性磷酸酶血癥，此癥狀被認為和惡性膽管阻塞、原發(fā)性膽管硬化、原發(fā)性硬化膽管炎、肝淋巴瘤和肝肉瘤等肝膽疾病密切相關；? ALP是成骨細胞的副產(chǎn)物，血清中ALP活性升高和骨頭發(fā)育密切關聯(lián)；? 血清ALP活性過低也與一些疾病相關；? 兒童和孕婦血清內ALP活性較常人高一些；血清中ALP活性范圍在20-140U/L。

檢測原理（堿性磷酸酶活性）：

對硝基苯酚磷酸鹽（pNPP）是一種常用的堿性磷酸酶（ALP）顯色底物，堿性條件下能被ALP催化生成對硝基酚（para-nitrophenol，p-nitrophenol），呈黃色產(chǎn)物，可在400-415nm檢測到吸光度。產(chǎn)物黃色越深，代表ALP活性越高，反之則酶活性越低。則通過比色分析可定量檢測ALP水平。反應式如下：



檢測對象：細胞或組織裂解液或勻漿液、血清、血漿、尿液等樣品內源性堿性磷酸酶活性。
 

訂購信息：Anaspec國*，>幾十篇文獻引用，高口碑產(chǎn)品。

試劑盒組分：

   AS-72146 SensoLyte pNPP Alkaline Phosphatase Assay Kit.pdf
 

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引用文獻（Anaspec，AS-72146）：

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 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

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Anaspec SensoLyte pNPP ALP Assay Kit 堿性磷酸酶活性檢測

Anaspec SensoLyte pNPP ALP Assay Kit 堿性磷酸酶活性檢測